Figure 7.
DYRK1A-related kinase activity in brain regions of Ts65Dn and control mice with and without EGCG treatment. The area under the curve (AUC) of the the p-FAM-Woodtide fluorescence was measured from protein isolated from brain regions of treated and untreated mice. DYRK1A-related kinase activity was significantly higher in the cerebellum than the other brain areas. A reduction in kinase activity in trisomic as compared to euploid mice was only found in the cerebral cortex. ECGC treatment increased kinase activity in the hippocampus and cerebellum, with kinase activity in Ts65Dn + EGCG treatment significantly higher than Ts65Dn + PBS treated animals.