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. 2020 Jun 26;10:10418. doi: 10.1038/s41598-020-67425-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-200b-3p is transferred via exosomes. (a) Exosomes were isolated from the HCC cell lines and the expression levels of miR-200b-3p in the exosomes were measured. (b) HUVECs were cultured with or without exosomes isolated from HLE cell culture supernatants, and the ERG expression levels in HUVECs were investigated by Western blotting. Upper panel, representative immunoblot data from three independent analyses of HUVEC lysates. Lower panel, relative expression of ERG. (c) HLE cells were transfected with miR-200b-3p overexpression plasmid or control plasmid and the exosomes were isolated from the culture supernatants. miR-200b-3p expression levels in the exosomes were quantified by qRT-PCR. (d) HUVECs were cultured with exosomes isolated from miR-200b-3p-overexpressing HLE cell culture supernatants, and the ERG expression levels in HUVECs were investigated by Western blotting. Culture supernatants from HLE cells transfected with control plasmid were used as a control. Upper panel, representative immunoblot data from three independent analyses of HUVEC lysates. Lower panel, relative expression of ERG. *p < 0.01, **p < 0.001, ***p < 0.0001.