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. 2020 Jun 26;11:3258. doi: 10.1038/s41467-020-16984-1

Fig. 1. Microscopy screen for compounds that activate autophagy in human Control-1 NPCs and neurons.

Fig. 1

a–d Compound dose-effect on number of LysoTracker+ and CYTO-ID+ fluorescent vesicles in NPCs (8330-8-RC1), after 24 h treatment with rapamycin, OSI-027, AZD2014, and AZD8055 (+500 nM CQ). Data points correspond to mean number of fluorescent vesicles per nuclei ± SEM from n = 5 biological replicates. e NPCs (8330-8-RC1) treated with compounds for 24 h at a dose that promoted maximum vesicle formation without toxicity. Scale bars are 50 μm. Representative images from n = 5 biological replicates. Inserts correspond to zoomed-in images below (scale bars are 25μm). f Screening strategy followed. g Representative images of 5-week differentiated neurons (8330-8-RC1) treated with compounds for 24 h (+500 nM CQ). LysoTracker+ (top) and CYTO-ID+ (bottom) vesicles are shown in the background of MAP2 neuronal staining (n = 3 biological replicates). Scale bars are 25 μm. Source data are provided as a Source Data file.