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. 2020 Jun 26;11:3258. doi: 10.1038/s41467-020-16984-1

Fig. 7. Single-dose mTORi has sustained effect on tau phenotypes.

Fig. 7

a Assay to measure effect of mTORi 24h-treatment on tau levels and neuronal viability over a time-course of 20 days. b–i A152T neurons (6-week differentiated FTD19-L5-RC6) were treated with rapamycin, OSI-027 or AZD2014 for 24 h, followed by compound washout (red arrow). b–f Total tau (TAU5), P-tauS396 and the neuronal microtubule marker β-III-tubulin were measured by western blot over a period of 20 days post treatment. Representative blots are shown, and graph data points represent mean protein densitometry (bands within brackets) ± SEM for n = 3 biological replicates. Dotted lines correspond to vehicle-treated protein levels. g–i Time-course analysis of effect on neuronal viability. Data points represent average % viability relative to vehicle ± SEM for n = 3 biological replicates. Source data are provided as a Source Data file.