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. 2020 May 22;57(7):2944–2958. doi: 10.1007/s12035-020-01905-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Time course expression pattern of ciliary genes. a Venn-diagram of all upregulated genes day 0 vs day 35 and all “Syscilia” ciliary genes detected at day 0 or 35. b Gene set enrichment analysis (GSEA) of “Syscilia” ciliary genes in all detected 11,180 genes. FDR false discovery rate, ES enrichment score. c RNA-seq time course progression plot of the ciliopathy-related genes IFT57, BBS2, TMEM231, TCTN2. d qRT-PCR showing upregulation of the dyslexia and ciliopathy-related genes IFT57, BBS2, TMEM231, TCTN2 during neuronal differentiation. e Correlation plots of log₂ fold change in RNA-seq vs. log₂ fold change in RT-qPCR. r denotes Pearson correlation coefficient. c, d Mean ± SEM are displayed. c, d, e red: Experiment 1, blue: Experiment 2. Norm. normalized, rel. relative