Table 1.
ESI-MS/MS linear parameters for steroids
| Compound | m/z | tR (min) | LOD (pg/mL) | LOQ (pg/mL) | Slope | Intercept | R2 |
|---|---|---|---|---|---|---|---|
| Squalene | 411.3985 | 2.25 | 0.51 | 1.71 | 3.01E+04 | 1.89E+06 | 0.94 |
| Lanosterol | 427.3934 | 8.03 | 0.32 | 1.06 | 2.57E+06 | 1.79E+04 | 0.99 |
| 7 Dehydrocholesterol | 385.3465 | 9.83 | 0.19 | 0.62 | 4.99E+05 | 9.86E+05 | 0.90 |
| 24 Hydroxyholesterol | 403.3571 | 9.84 | 1.00 | 9.79 | 1.83E+05 | 2.74E+06 | 0.94 |
| 25 Hydroxycholesterol | 403.3571 | 8.91 | 2.63 | 3.57 | 4.07E+05 | 1.74E+06 | 0.99 |
| 27 Hydroxyholesterol | 403.3571 | 10.2 | 3.59 | 4.56 | 5.47E+05 | 8.11E+05 | 0.99 |
| Progesterone | 315.2319 | 7.48 | 2.94 | 3.34 | 4.98E+05 | 4.57E+06 | 0.91 |
| Corticosterone | 347.2217 | 5.93 | 1.07 | 8.76 | 4.94E+05 | 4.57E+06 | 0.99 |
| Testosterone | 289.2162 | 6.53 | 1.37 | 11.96 | 3.78E+05 | 1.77E+06 | 0.91 |