Table 2.

Intraday, interday error and recovery of the LC-MS method

Compound	Intraday precision (n = 5)	Interday precision (n = 5)	Recovery (%)
	ng ± SD (%RSD)	ng ± SD (%RSD)
7-Ketocholesterol (d7)	1.938 ± 0.16 (8.29)	1.85 ± 0.17 (9.15)	81.73
Squalene	0.544 ± 0.07 (12.97)	1.106 ± 0.083 (7.52)	91.93
Lanosterol	1.049 ± 1.08 (11.97)	2.366 ± 0.30 (13.03)	88.61
7 Dehydrocholesterol	0.780 ± 0.05 (6.80)	2.011 ± 0.28 (14.01)	94.18
24 Hydroxyholesterol	1.268 ± 0.06 (5.37)	1.105 ± 0.09 (8.27)	84.92
25 Hydroxycholesterol	1.077 ± 0.05 (5.12)	1.75 ± 0.20 (11.68)	86.25
27 Hydroxyholesterol	1.267 ± 0.15 (11.99)	0.584 ± 0.071 (12.15)	78.53
Progesterone	0.465 ± 0.46 (2.29)	1.217 ± 0.12 (10.31)	73.82
Corticosterone	1.097 ± 1.16 (10.7)	0.816 ± 0.083 (10.26)	93.81
Testosterone	0.915 ± 0.12 (13.59)	1.961 ± 0.15 (7.83)	81.00