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. 2020 May 25;57(7):2996–3013. doi: 10.1007/s12035-020-01937-y

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© The Author(s) 2020

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Fig. 7 — Effects of mild mitochondrial uncoupling on the enrichments of miRNAs in mitochondria and MAM fractions. The effects of different concentrations of uncoupling agent 2,4-dinitrophenol (DNP) on mitochondria-enriched miRNAs (a) and on the miRNAs that were not enriched in mitochondria (b) (n = 7 of rats in each group. One-way ANOVA and 2-sample unpaired Student’s t test). The relative quantity (RQ) of miRNAs in each group was expressed as RQ = 2^{−(Ct-Mito − Ct-MAM)} (RQ equals 2 to the power of negative (Ct-Mito minus Ct-MAM). Vehicle: DMSO; DNP2.5: 2.5 mg/kg DNP treatment; DNP5: 5 mg/kg DNP treatment