Table 2.
MAM-associated miRNAs are bound in AGO complexes
| Human cortical tissue | ||||||||
| Case 3 | Case 5 | |||||||
| MAM | pCyto | MAM | pCyto | |||||
| IPs | AGO | NMS | AGO | NMS | AGO | NMS | AGO | NMS |
| miR-107 | 151.8 | 1.0 | 1183.8 | 1.0 | 3.0 | 1.0 | 25.6 | 1.0 |
| miR-142-3p | 16.5 | 1.0 | 766.0 | 1.0 | 7.7 | 1.0 | 62.4 | 1.0 |
| miR-142-5p | 14.3 | 1.0 | 370.4 | 1.0 | 10.3 | 1.0 | 4.2 | 1.0 |
| miR-146a | 155.9 | 1.0 | 158.7 | 1.0 | 36.9 | 1.0 | 67.6 | 1.0 |
| miR-223 | 163.1 | 1.0 | 890.7 | 1.0 | 29.0 | 1.0 | 68.4 | 1.0 |
| Rat cortical tissue | ||||||||
| Rat 1 | Rat 2 | |||||||
| MAM | pCyto | MAM | pCyto | |||||
| IPs | AGO | NMS | AGO | NMS | AGO | NMS | AGO | NMS |
| miR-107 | 214.7 | 1.00 | 549.1 | 1.00 | 3.2 | 1.0 | 30.8 | 1.0 |
| miR-142-3p | 77.3 | 1.00 | 223.2 | 1.00 | 9.8 | 1.0 | 64.3 | 1.0 |
| miR-142-5p | 32.6 | 1.00 | 8.2 | 1.00 | 8.7 | 1.0 | 34.5 | 1.0 |
| miR-146a | 631.2 | 1.00 | 328.3 | 1.00 | 56.2 | 1.0 | 43.1 | 1.0 |
| miR-223 | 90.1 | 1.00 | 662.6 | 1.00 | 36.0 | 1.0 | 56.8 | 1.0 |
Single-tube TaqMan® miRNA analysis of AGO and NMS IP products from MAM and cytosol fractions. The relative miRNA levels in AGO-IP were expressed as fold change relative to NMS-IP in the same fraction (mitochondria-associated ER membrane fraction (MAM) or cytosol (pCyto)). The fold change was calculated using the following equation: fold change = 2 − (Ct-AGO-IP − Ct-NMS-IP) (fold change equals 2 to the power of negative (Ct-AGO-IP minus Ct-NMS-IP)