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Michaelis-Menten constants for phLDH and rmLDH with free NADH as substrate:.
| PhLDH | rmLDH | |
|---|---|---|
| Measurement type: | ||
| Vmax, units per milligrama | 130 ± 20 | 140 ± 20 |
| KM, μMb | 7.8 ± 0.9 | 4.4 ± 0.4 |
aone units is equivalent of 1 micromole NADH oxidized per minute; bMeasured Michaelis-Menten parameters depend on pyruvate concentrations, we measured phLDH and rmLDH activity in the presence of 630 μM pyruvate.
