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. 2020 Jun 26;10:10404. doi: 10.1038/s41598-020-67079-2

Table 3.

Apparent Michaelis-Menten constants for phLDH and rmLDH with rmGAPDH-NADH or byGAPDH-NADH complex as substrate:.

phLDH rmLDH
Measurement type: rmGAPDH-NADH rmGAPDH-NADH
Vmax, units per milligrama 6.5 ± 0.4 8.2 ± 0.7
KM, μMb 59.6 ± 6 35 ± 7
Measurement type: byGAPDH-NADH byGAPDH-NADH
Vmax, units per milligrama 192 ± 10 241 ± 3
KM, μMb 116 ± 8 78 ± 2

aone units is equivalent of 1 micromole NADH oxidized per minute; bMeasured Michaelis-Menten parameters depend on pyruvate concentrations, we measured phLDH and rmLDH activity in the presence of 630 μM pyruvate.