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. 2020 Jun 26;11:3231. doi: 10.1038/s41467-020-17030-w

Fig. 3. miR-181a increases nuclear rupture and mitotic/cytokinetic defects in FTSECs.

Fig. 3

a Immunofluorescence micrographs of representative DAPI stained nuclei (top), matched circularity masks with circularity value displayed in upper right hand corner (middle), and graph showing the circularity distribution (bottom) from pscram-miR, pmiR-181a, and pmiR-181a + antimiR cells. b Time-lapse micrographs of pscram-miR cells (left) and a nuclear membrane rupture event in pmiR-181a cells (right) expressing SV40-GFP. White lines indicate the outline of the cell in each micrograph. c Quantification of the percent of cells with nuclear membrane rupture for the pscram-miR, pmiR-181a, and pmiR-181a + antimiR cells. N = 5 for each cell line, ≥80 cells measured for each cell line. d Floating bar plot of the number of nuclear ruptures/cell for the pscram-miR, pmiR-181a, and pmiR-181a + antimiR cells. N = 5 for each cell line, ≥80 cells measured for each cell line. e Box and whisker plot with distribution curves of the cell division times for the pscram-miR, pmiR-181a, and pmiR-181a + antimiR cells. N = 3 for each cell line, 60 cell divisions measured for each cell line. f Representative time-lapse micrographs of mitotic and cytokinetic defects in pmiR-181a cells expressing H2B-GFP with quantification below. White outlines in the NP bridge and Lag. Chrom. panels denote the outline of the cell in each image. N = 4 for each cell line. g Cell fate outcomes for pscram-miR, pmiR-181a, and pmiR-181a + antimiR cells with before and after plot (left) of cell division and daughter cell fate outcomes and percentage quantification of daughter cell fate (right). Color key for cell division and daughter fate outcomes are on right. N = 100 cell divisions were observed for each cell line. Chi-square analysis for statistical comparison between groups. All data are representative of N = 3 independent experiments unless otherwise stated. The measure of center for the error bars is given as the mean value unless otherwise stated. Two-sided Student’s t test was used unless otherwise stated. Error bars indicate ±standard deviation unless otherwise stated. N.R. nuclear rupture. *p < 0.05, **p < 0.005, ***p < 0.0005.