Figure 5.

Missing-wedge correction of the NS single-molecule 3D reconstructions. (A) Two perpendicular views of a single-molecule 3D density map (low-pass filtered at 8 Å) of an individual CETP particle reconstructed by IPET on the NS tilt series (a portion of tilt series from −45° to +45° in steps of 1.5° was used for 3D reconstruction) after CTF correction and contrast enhancement²⁵. (B) The corresponding views of the 3D map after the 1,000 iterations of missing-wedge correction. (C) The corrected 3D density map rigid-body-docked with the crystal structure of CETP. (D,E) Fourier transforms of X-Z projections of the initial and final 3D maps. (F–I) The missing-wedge correction on a 3D map of an individual complex of DNA-nanogold conjugate imaged by NS ET and reconstructed by IPET from a portion of the tilt series (from −45° to +45° in steps of 1.5°). Two perpendicular views and Fourier transforms of the X-Z projections of the 3D maps before and after correction are shown. (J–M) The missing-wedge correction on a 3D map of an individual nucleosome particle (DNA-histone complex) imaged by NS ET and reconstructed by IPET from a portion of the tilt series (from  −45° to +45° in steps of 1.5°). Two perpendicular views and Fourier transforms of X-Z projections of the 3D maps before and after correction are shown. Scale bars: 5 nm in A and 20 nm in H and L.