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Phospholipase C-β2 (PLC-β2)-positive type II and carbonic anhydrase 4 (CA4)-positive type III taste cells in cultured taste organoids. (A) A bright-field image of an organoid. (B, C) Immunostaining of an organoid (B) and a mouse circumvallate section (C) with antibodies against PLC-β2 (green) and CA4 (red), showing separate populations of types II and III taste cells. Scale bars: 40 µm.
