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TLR mRNA expression in organoids derived from mouse circumvallate stem cells. (A) Amplification curves from qRT-PCR of mouse TLRs and β-actin, showing readily detectable expression of several TLRs. (B) Agarose gel image of DNA products from RT-PCR of TLRs 1–6 and β-actin. M: 1 Kb Plus DNA Ladder (Invitrogen). Technical repeats from one of two independent experiments are shown.
