Table 2.

Mutations detected by GenoType MTBDRplus assay in rifampicin or isoniazid resistant Mycobacterium tuberculosis.

S. No	Band Missing (WT a)/Mutation present (MUT b)	Gene Region/specific mutation rpoB	MDR e (n = 94)	Mono RIF f resistant (n = 43)	Total RIF resistant (n = 137)	Mono INH g resistant (n = 40)	Total INH resistant (n = 134)	P value
1	rpoB
2	WT1	506–509	0	1	1			–
3	WT2	510–513	7	4	11			–
4	WT4	517–519	2	0	2			–
5	WT1,WT4	506–509,517–519	2		2
6	WT3,WT4	513–517,517–519	9	2	11			–
7	WT1, WT5,WT6	506–509, 518–522,521–525	0	1	1
8	WT7	526–529	3	0	3			–
9	WT8	530–533	5	1	6			–
10	UKc		28	9	37			0.3
11	MUT 1	D516 V	6	2	8			1
12	MUT2A	H526Y	5	2	7			1
13	MUT2B	H526D	4	1	5			1
14	MUT3	S531 L	51	29	80			0.2
15	Total		94	43	137
16	katG
17	WT	315	5			3	8	–
18	UKc		5			3	8	–
19	MUT1	S315T1	81			29	110	0.08
20	MUT2	S315T2	1			0	1	–
21	Total		87			32	119	0.07
22	inhA
23	WT1	15/16	1			0	1	–
24	WT2	8	0			0	0	–
25	UKc		1			0	1	–
26	MUT1	C15T	8			9	17	0.04
27	MUT2	A16 G	0			0	0	–
28	MUT3A	T8C	2			1	3	–
29	MUT3B	T8A	0			0	0	–
30	Total		11			10	21	0.06

(−) p value not calculated due to insignificant numbers.

S. No 2–10, 17,18, 23–25 denote mutations due to missing wild type without specific mutation.

S. No 11–14, 19, 20, 26–29 denote specific mutations.

S. No 15, 21 and 30 denote total mutations in rpoB, katG and inhA genes respectively.

^dMDR: Multi-drug resistant; resistant to both rifampicin & isoniazid.

^aWT: Wild type.

^bMUT: Mutant.

^cUK: No known mutations as defined by the kit.

^eRIF: Rifampicin.

^fINH: Isoniazid.