Figure 6.

WFDC2 expression in human placenta samples over gestation. (A–D) Human blastocyst developmental age 16 days. WFDC2 most strongly associates with primitive syncytium high in CGA and low in KRT7 staining. (E and F) Six weeks 6 days human placenta was immunostained for STB marker CGA (E, G) and CTB/EVT marker KRT7 (F, H) in floating (E, F) and anchoring (G, H) villi. (I–O) are placental villi from the first trimester at gestational ages (I) 5 weeks 5 days, (J) 6 weeks 6 days, (K) 7 weeks 6 days, (L) 8 weeks 2 days, (M) 13 weeks, (N) 20 weeks 6 days and (O) Term. Line arrows indicate WFDC2+ anchoring villi. (P) CTCF quantification of positive CTB/STB regions. First trimester samples showed higher fluorescence intensity values than second trimester and term. Best fit line is y = x + x². R² = 0.61. Scale bars = 100 µm.