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. 2020 May 19;27(2):dsaa009. doi: 10.1093/dnares/dsaa009

Figure 7.

Figure 7

Phylogenetic analysis of the candidate transcripts annotated as cytochrome P450s in Cornus officinalis together with CYPs representing major-classes of plant CYPs. Seven transcripts (represented with a black circle) were highly correlated with at least 50% of assigned iridoid biosynthetic genes as well as with at least one iridoid of a total 15 being identified in this study. Nucleotide sequences were translated to their corresponding protein sequences and aligned using MUSCLE programme. The phylogenetic tree was inferred using the Maximum Likelihood method with bootstrap values obtained after applying 1,000 replications using the MEGA X programme. Bootstrap values above 60% are shown here. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Evolutionary analyses were conducted in MEGA X. At, Arabidopsis thaliana; Os, Oryza sativa; Cj, Coptis japonica; El, Euphorbia lagascae; Tc, Taxus cuspidata; Nt, Nicotiana tabacum; Aa, Artemisia annua; Zz, Zingiber zerumbet; Cr, Catharanthus roseus; Gu, Glycyrrhiza uralensis; Hc, Hypericum calycinum; Ph, Petunia x hybrida.