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. 2020 May 31;25(11):2562. doi: 10.3390/molecules25112562

Figure 1.

Figure 1

Transient expression of mistletoe lectin II (ML-II) in Nicotiana benthamiana via pEAQ-HT cloning. (a) Schematic representation of the constructs expressing native ML-II with a C-terminal His-tag and ML-II with the KDEL retention signal using the pEAQ-HT vector. The signal peptide (SP) and the His-tag (His) on the ML-II sequence are indicated in white boxes. The cauliflower mosaic virus (CaMV) 35 promoters are indicated by green arrows, whereas nopaline synthase and CaMV 35S terminators are indicated by white boxes. RB and LB represent the left and right T-DNA borders, respectively; (b) ELISA measuring the expression levels of the two constructs at 7 dpi; (c) Expression profile of the ML-II KDEL protein in Nicotiana benthamiana. SDS-PAGE and Western blot assessment of the total soluble protein extracted at days 3, 5, 7, 9, and 11 post infiltration, respectively. Lane 1, M protein molecular weight marker. ML-II was detected using KPL’s HisDetector. The black arrow indicates the whole protein under nonreducing conditions (50 kDa), with the red arrow showing the B chain under reducing conditions (30 kDa).