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. 2020 May 31;25(11):2562. doi: 10.3390/molecules25112562

Figure 3.

Figure 3

Characterization of plant produced ML-II. (a) SDS-PAGE of ML-II in nonreducing and reducing conditions showing the whole protein and single A (25 kDa) and B (30 kDa) chains; (b) SDS-PAGE with Coomassie brilliant blue and periodic acid-Schiff stained gel. Lane 1, HIV envelope glycoprotein 120 (gp120, highly glycosylated protein), Lane 2, bovine serum albumin (BSA) (a non-glycosylated protein), and Lane 3, purified ML-II; (c) Isoelectric focusing (IEF) of purified ML-II. Lane M, IEF marker 3–10 PI; (d) Carbohydrate-binding activity of ML-II by enzyme-linked lectin assay (ELLA) using asialofetuin (ASF). The EC50 of ML-II was determined to be 2.98 μg/mL.