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. 2020 May 31;25(11):2562. doi: 10.3390/molecules25112562

Figure 4.

Figure 4

ML-II inhibits the growth of two human lung cancer cell lines in vitro. (a) ML-II treatment of H460 and A549 cells resulted in altered cell morphology. Cells were treated with vehicle (PBS) or ML-II (2, 10, 20, and 50 µg/mL) for 24–96 h and observed under an inverted EVOS microscope (10×). Images are representative of three independent experiments performed in triplicate (bar, 400 μm); (b) Cell viability measured by the 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay after 96 h of incubation with ML-II or vehicle (PBS). Cells were plated in 96-well plates and treated with vehicle or ML-II, starting at a concentration of 50 µg/mL and 1/3 dilution. After 96 h, cells were incubated with the MTS reagent, and absorption was read at 490 nm. The EC50 values for H460 and A549 cells were determined to be 4 and 3.5 µg/mL, respectively. Data are expressed as percentage of the control (considered 100%), and values were presented as mean ± SD.