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. 2020 May 31;25(11):2562. doi: 10.3390/molecules25112562

Figure 5.

Figure 5

ML-II causes lung cancer cell apoptosis. (a) Flow cytometry data after annexin V-448Aand PI double staining of human H460 cells treated with ML-II (10 µg/mL); (b) Flow cytometry data after annexin V-448A and PI double staining of human A549 cells treated with ML-II (10 µg/mL). The vehicle (PBS) was used as a control, and percentages of stained cells were assessed at 24, 48, and 72 h after treatment. The right lower quadrant represents annexin V-448A positive/propidium iodide (PI) negative staining (Q1-LR: AV+/PI−) indicating early apoptosis; the right upper quadrant represents both high annexin V-448A and PI staining (Q1-UR: AV+/PI+) indicating late apoptosis; the left upper quadrant represents low annexin V-448A, and high PI staining (Q1-UL: AV−/PI+) indicating necrosis; and the left lower quadrant (Q1-LL: AV−/PI−) indicates viable cells.