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. 2020 May 31;25(11):2562. doi: 10.3390/molecules25112562

Figure 6.

Figure 6

ML-II-induced apoptosis is reduced by incubation with d-galactose. Flow cytometry data after annexin V-448A and PI double-staining of H460 cells administered vehicle (PBS), PBS + 0.1 M galactose, ML-II (10 µg/mL), and ML-II (10 µg/mL) + 0.1 M d-galactose. Percentages of annexin V-448A positive cells were assessed after 24 h. Groups were analyzed by one-way ANOVA using Bonferroni’s multiple comparison test (p < 0.0001). Asterisks (****) indicate statistically significant differences (p < 0.0001) for the vehicle group vs. ML-II group and ML-II group vs. ML-II + 0.1 M galactose group. ML-II + 0.1 M galactose vs. ML-II showed >50% apoptosis reduction.