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. 2020 May 27;25(11):2498. doi: 10.3390/molecules25112498

Table 1.

Characteristic Raman and infrared bands for proteins [33,34]

Amide Bands Wavenumber [cm−1] Assigned Vibration 1
A 3500 ν(N-H)
B 3100 ν(N-H)
I 1700–1600 80% ν(C=O), 10% ν(C-N), 10% δ(N-H)
II 1580–1480 40% ν(C-N), 60% δ(N-H)
III 1300–1230 30% ν(C-N), 30% δ(N-H), 10% ν(CH3-C), 10% δ(O=C-N), 20% other
IV 770–625 40% δ(O=C-N), 60% other
V 800–640 γ(N-H)
VI 600–540 γ(C=O)
VII 200 skeletal mode

1 ν—stretching, δ—bending, γ—out-of-plane bending