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. 2020 May 31;25(11):2564. doi: 10.3390/molecules25112564

Figure 4.

Figure 4

Combined results obtained with SDS-PAGE, Western blot and protonography, which were used to evaluate the recombinant CynT2 product purified by affinity column. The yellow band evidenced in the protonogram corresponds to the enzyme activity responsible for the drop of pH from 8.2 to the transition point of the dye in the control buffer. Lane STD, molecular markers (form bottom to the top: 20, 25, and 37 kDa); Lane 1, SDS-PAGE; Lane 2, Western Blot; Lane 3, Protonogram; Molecular markers (Lane STD).