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. 2020 May 28;13(11):2453. doi: 10.3390/ma13112453

Figure 3.

Figure 3

Analysis of ORC-dependent local pH in PBS, DMEM, and CSF determined with pH-sensitive fluorescence imaging and microscopy. (A) 5 cm diameter petri dish with SNARF in the medium and 95 mm² Tabotamp® in the middle. The intensity distribution of the neutral spectrum is shown at the top and the acidic spectrum at the bottom. The colors were set with the Histogram eq. mode and the color map “jet”. The scale is in parts of the maximum intensity. The blue stands for low intensity and red for high intensities. The knitting structure is shown in orange. (B) Fluorescence microscope picture of Tabotamp® in CSF. The left side shows the neutral and the acidic spectrum distribution. The right side presents the calculated pH as described in methods. The pH was calculated with a sigmoidal calibration curve (Figure S2).