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. 2020 May 29;25(11):2545. doi: 10.3390/molecules25112545

Figure 1.

Figure 1

(A) Increase of fluorescence (λex = 501 nm, λem = 525 nm) of solutions containing a mixture of 2′,7′-dichlorofluorescin (10 μM), 3-(N-morpholino)propanesulfonic acid (MOPS, 100 mM, pH 7.5), N,N,N′,N′-ethylenediaminetetracetic acid (EDTA, 10 mM), glutathione (GSH, 5 mM), H2O2 (10 mM, curve 1, negative control) and either FeSO4 (49.5 μM, curve 2, positive control) or prodrug 8 (49.5 μM, curve 3) or prodrug 5 (49.5 μM, curve 4); (dF/dt)0 = 22.3, 17.1, 7.7 and 0.1 a.u. × min−1; temperature = 37 °C. (B) Increase of fluorescence (λex = 551 nm, λem = 576 nm) of solutions containing a mixture of prodrug 7 (0.5 μM), phosphate-buffered saline (10 mM phosphate, 150 mM NaCl, pH 7) and either without (curve 1, negative control) or with H2O2 (50 mM, curve 2). (dF/dt)0 = 0.5 (curve 1), 2.9 (curve 2) a.u. × min−1.