Skip to main content
. 2020 May 29;25(11):2545. doi: 10.3390/molecules25112545

Figure 3.

Figure 3

(A) Accumulation of prodrugs 5, 9 and 10 (each 1 μM, incubation time- 1 h, temperature- 37 °C) in Burkitt’s lymphoma BL-2 cells monitored by analysis of the intracellular concentration of boron (Table S3, SI). (B) Uptake of fluorescent prodrug 7 (0–25 μM, incubation time—2 h, temperature—37 °C) by BL-2 cells monitored by flow cytometry (λex = 488 nm, λem= 530 nm). (C) Monitoring ROS-release in BL-2 cells (using 5(6-)chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate as a fluorogenic probe, 10 μM) in the presence of prodrugs 5, 7, 9 as well as controls 11 (negative control) and a mixture of FeCl3 with 8-hydroxyquinoline (HQ) with a molar ratio of components correspondingly 1 to 2 (Fe(HQ)2, positive control); incubation time—2 h, temperature—37 °C. Student’s t-test: **—p < 0.01; ***—p < 0.001.