(A) Effect of prodrug 5 and controls 9 and 12 used at the indicated on the plot concentrations on the viability of Jurkat cells (incubation time: 48 h). The cells were stained and analyzed by flow cytometry as described in the SI. Annexin A5-FITC (Ax) and propidium iodide (PI) negative cells (indicated “Ax-/PI-“) were considered viable, “Ax+/PI-“—apoptotic and “Ax+/PI+” necrotic. (B) Effect of prodrug 5 and controls 9 and 12 used at the indicated on plot concentrations (incubation time: 48 h) on mitochondrial membrane potential of Jurkat cells. Fluorescence intensity corresponding to the potential-sensitive dye 1,1′,3,3,3′,3′-hexamethylindodicarbocyanine iodide (DiIC1(5)) is plotted on the Y-axis (λex = 638 nm; λem = 655-695 nm). Student’s t-test: **—p < 0.01; ***—p < 0.001.