Table 2.
TIRF fluctuation analysis of plasma membrane-associated LRRK2-GFP.
| Transfected Protein(s) | Concentration (nM) | Average Brightness (cpsm) | Norm. Brightness |
Puncta Variance (sec) |
|---|---|---|---|---|
| mGFP | 100 ± 10 | 1.10 ± 0.03 | 1.0 | N/A |
| WT LRRK2-GFP |
55 ± 15 | 1.33 ± 0.08 | 2.5 | 55 ± 15 |
| G2019S LRRK2-GFP |
90 ± 30 | 1.33 ± 0.10 | 2.5 | 25 ± 10 |
Average brightness (ε) is reported as counts per second per molecule. Normalized brightness values were generated by dividing the brightness of the sample over the monomer control. Puncta variance refers to the average time spent on the plasma membrane. Error is reported as ± standard deviation. n > 20 cells per condition.