Figure 3.

CM544 modulates enzyme of the glycolytic pathway. BV2 microglial cells were pre-treated with 10 μg/mL LPS for 3 h and then 200 μM CM544 was added for further 1 h, 2 h, 3 h, and 21 h. At each time point, cells were collected, and total extract used to analyze the enzyme of the glycolytic pathway by Western blotting. β-actin was used as loading control. Numbers above the images represent the ratio between respective protein and β-actin band intensity. The images are representative of one out of three separate experiments.