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. 2020 Jun 6;25(11):2641. doi: 10.3390/molecules25112641

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Flow cytometric analysis of E. coli CECT 434 treated with different EO compounds. Bacterial cell samples were stained with a mixture of Syto^® 9 and PI. The Figure shows different staining patterns of (A) untreated; (B) eugenol-treated; (C) trans-cinnamaldehyde-treated; (D) citronellol-treated; and (E) terpineol-treated cell samples. UL (upper-left), Syto^® 9+PI– and healthy cells; UR (upper-right), Syto^® 9+PI+ and moderately damaged cells; LR (lower-right), Syto^® 9–PI+ and severely damaged cells; LL, (lower-left), Syto^® 9–PI– and unknown cells.