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. 2020 Jun 5;25(11):2637. doi: 10.3390/molecules25112637

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The use of small interfering RNA for guanine deaminase (siGDA) in keratinocytes (KCs) downregulates melanogenesis while guanine deaminase (GDA) overexpression promotes melanogenesis in the coculture. (A) Melanin content and (B) GDA and tyrosinase mRNA expression levels were decreased by siGDA in the coculture of normal human melanocytes (NHMs) and normal human keratinocytes (NHKs) for 5 d. (C) While UVB increased melanin content in the negative control siRNA (siNC)-transfected coculture of NHMs and NHKs, siGDA effectively reversed this accelerated melanin accumulation by UVB on day 5. (D) Melanin content was increased by a lentiviral-overexpressed GDA gene in the coculture of NHMs and HaCaT cells on day 5. * p < 0.05, ** p < 0.01.