Figure 2.

¹H-NMR measurements of Tau^4RD monomer depletion and translational diffusion. High-field region of ¹H-NMR spectra (A) and translational diffusion experiments (B) recorded on Tau^4RD in the presence of OLA. High-field region of ¹H-NMR spectra (C) and translational diffusion experiments (D) recorded on Tau^4RD in the presence of ARA. Samples contained 25 μM Tau^4RD and 75 μM fatty acid. Spectra in (A,C) were collected on samples that were incubated for 0 h (gray), 24 h (dark gray), and 48 h (black). In (B,D), the normalized intensity of a selected signal is shown as a function of the square of the fraction of gradient used; errors in peak intensities were smaller than the size of the symbols, as estimated from duplicate experiments; samples were incubated for 0 h (empty circles/diamonds), 24 h (gray filled circles/diamonds), and 48 h (black filled circles/diamonds); asterisks denote data collected on Tau^4RD in the absence of fatty acid; lines are fitted exponential curves. Protein samples were aliquots taken from 100 μM protein solutions incubated with 300 μM fatty acid under magnetic stirring. Samples were kept at 37 °C during both incubation and measurement.