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. 2020 Jun 2;13(11):2530. doi: 10.3390/ma13112530

Figure 2.

Figure 2

Verification of Cs-g-PNIPAAm composition via (a) 1H-NMR spectra of NIPAAm monomer, PNIPAAm, Chitosan in 1% acetic acid, and Cs-g-PNIPAAm copolymer; (b) comparison of Cs in acetic acid and Cs-g-PNIPAAm copolymer. 1H-NMR confirmed presence of PNIPAAm and chitosan in the structure. Lack of peaks between 5.5–6.5 ppm demonstrates no monomers in the copolymer. (c) The ζ-potential of Cs and Cs-g-PNIPAAm with 10%, 20%, and 30% Cs in relation to PNIPAAm after reconstitution in different media (DDW, Tris-EDTA (TE) buffer, PBS).