Figure 5.

Deficiency of UFL1 affects milk protein synthesis in the mammary. (a–d) The lactation of the mammary gland was collected from UFL1 KO mice and WT mice as previously described. (a) After samples were harvested, western blot was used for analyzing milk protein synthesis-related proteins CSN2, ACACA, and FASN levels. The relative intensity of (a) was plotted in (b–d). (e–h) HC11 cells were transfected with control siRNA and vehicle (control) as previously described. (e) The levels of CSN2, ACACA, and FASN were determined by western blot. The relative intensity of (f) was plotted in (f–h). Data were analyzed by t-test with representative of 3 times independent experiments. Asterisks indicate significant difference (P < 0.05).