Figure 5.

MicroED radiation damage and experimental phasing.

(a) Disulfide bonds of the proteinase K structures determined from data collected on Falcon III and CetaD. The 2mFo-DFc densities (blue meshes) are contoured at 1.5 σ (Reprint of Ref. [63^••]). (b) Fourier difference maps between the damaged and undamaged structure of a peptide, contoured at 3 σ (Top left). Maps of the experimental phases of the peptide extended to 1.4 Å (Top right). Maps of an intermediate model-building step (Bottom left) to generate the final peptide structure at 1.4 Å (Bottom right) (Reprint from Ref. [64^••]).