Skip to main content
. 2020 Jun 23;11(25):2387–2403. doi: 10.18632/oncotarget.27630

Figure 3. Metaphase arrest by the treatment of BI-D1870.

Figure 3

(A) HL60 cells were synchronized at metaphase using a thymidine plus nocodazole block. One hour before release, cells were treated with BI-D1870 (5 μM). Cells were released from the nocodazole block (metaphase arrest) and collected at the indicated times with or without BI-D1870 (5 μM). Flow cytometry profiles show the bivariate distribution of expression of Cyclin B versus expression of Cyclin A or Securin in p-H3-positive mitotic cells. The transition from prophase to ana/telophase is characterized by the cellular levels of Cyclin A, Cyclin B, and Securin. Cells started to enter anaphase by 1 h after release. G1 phase population was identified by DNA content and protein levels of p-H3, Cyclin A, and Cyclin B. Plots represent one out of three experiments. Percentages of cell populations residing at metaphase (B) or G1 phase (C) were calculated using FlowJo software and are expressed as mean ± SEM (n = 3).