Figure 3. Reversible effects of a talin modulator. (A) HAoSMCs were treated with a talin modulator for 24 hours and then cultured in fresh media for 24, 48, and 72 hours. Scale bars represent 100 μm. (B, C) Western blot analyses of FAK, MEK1/2, ERK1/2, and AKT phosphorylation after treatment, and at 2 and 4 hours after wash out of the talin modulator. All band intensities were normalized to GAPDH, and the phosphorylated forms were then normalized to the total form of the respective forms.

AKT = protein kinase B; ERK1/2 = extracellular signal-regulated kinase 1/2; FAK = focal adhesion kinase; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; HAoSMC = human aortic smooth muscle cell; MEK1/2 = mitogen-activated protein kinase kinase 1/2.

^*p<0.05 vs. treatment. All experiments were repeated at least 3 times independently.