Figure 1.

Effect of Various Concentrations of 10 Essential Metal Ions on the Transduction Efficiency of AAV2 Vectors

HeLa cells were grown in DMEM supplemented with serum KO for at least three passages before being plated in a 96-well plate and transduced with scAAV2-CBA-EGFP at an MOI of 200 vg/cell. (A–J) Cells treated 1 h after transduction with various concentrations of each of the 10 essential metal ions separately in triplicate (A, calcium; B, cobalt; C, copper, D, iron; E, magnesium; F, manganese; G, molybdenum; H, potassium; I, sodium; J, zinc). Transgene expression was determined 48 h post-transduction as described under Materials and Methods. Values represent fold increase in mean AFU, gathered 48 h post-transduction, compared to controls. Means ± SD are plotted. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005, based on Student’s t test of the means.