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. 2020 Jun 1;21:205–216. doi: 10.1016/j.omtn.2020.05.029

Figure 4.

Figure 4

The Efficiency of ASOs in 2′-MOE Backbone in Pseudo-Exon Skipping at RNA Level

(A) Representative picture of PCR products from fibroblasts treated with ASO-5, ASO-6, ASO-5/6, or ASO-9 in 2′-MOE backbone for 24 h with Lipofectamine transfection. (B) Quantitative real-time PCRs of total COL6A1 and mutant COL6A1 transcripts were performed in RNA samples collected from four patient skin fibroblasts treated with ASO-5, ASO-6, ASO-5/6, or ASO-9 at concentrations ranging from 2.5 to 20 nM. Data are presented as mean ± SD. Data were analyzed by one-way ANOVA and post-Bonferroni test (∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗∗p ≤ 0.0001).