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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: Cancer Cell. 2020 Feb 10;37(2):200–215.e5. doi: 10.1016/j.ccell.2020.01.001

Figure 1. MYC Interacts with AURKB.

Figure 1.

(A) Purification of MYC and its interacting partners. Total protein extract from Jurkat cells expressing Flag-tagged MYC or vector was subjected to immunoprecipitation using anti-Flag beads. Eluted proteins were resolved by SDS-PAGE and visualized by silver staining.

(B) Liquid chromatography-tandem mass spectrometry analysis of the Flag-MYC-associated peptides corresponding to AURKB.

(C) Lysates of 293T cells overexpressing Flag-AURKB and/or HA-MYC were subjected to reciprocal co-immunoprecipitation (co-IP) to detect protein interaction.

(D) CUTLL1 and HPB-ALL cell lysates were subjected to co-IP and immunoblot to detect endogenous MYC and AURKB interaction.

(E) Schematic presentation of various human MYC truncations used in AURKB-binding assays (left). Lysates from 293T cells overexpressing Flag-AURKB and/or various HA-MYC truncations were subjected to co-IP and immunoblot (right).

(F) Co-IP of HA-AURKB and Flag-tagged streptavidin-binding peptide (SBP)-fused MYC PEST domain (left), or Flag-AURKB and HA-MYCΔPEST (right) from lysates of 293T cells overexpressing respective tagged proteins.

See also Table S1.