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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: Cancer Cell. 2020 Feb 10;37(2):200–215.e5. doi: 10.1016/j.ccell.2020.01.001

Figure 2. AURKB Inhibition Attenuates the MYC Protein Accumulation and Transcriptional Activity.

Figure 2.

(A) AURKB was depleted by specific shRNAs in T-ALL cells as indicated. AURKB and MYC proteins were analyzed by immunoblot, with ACTIN as a loading control. shGFP, control shRNA targeting GFP.

(B) Immunoblots of MYC and ACTIN in T-ALL cells treated with 10 nM AZD1152 for 24 h.

(C) Time-course analysis of MYC protein levels in AURKB-depleted CUTLL1 cells. MYC proteins were quantified and plotted on the right.

(D) AURKB-depleted CUTLL1 and KOPTK1 cells were treated with MG132 (10 μM) for 6 h before harvest. AURKB and MYC were analyzed by immunoblot, with ACTIN as a loading control.

(E) A heatmap depicting top 30 down- and upregulated genes upon AURKB depletion in CUTLL1 cells (Q < 0.05).

(F) qRT-PCR analysis of representative MYC-induced target genes in CUTLL1 cells upon AURKB depletion. Data shown represent the means (±SD) of biological triplicates. *p < 0.05, **p < 0.01, unpaired two-tailed Student’s t test.

(G) Gene set enrichment analysis of MYC target gene sets in the expression profiles of CUTLL1 cells expressing AURKB shRNA (http://software.broadinstitute.org/gsea).

See also Figure S1.