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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: Cancer Cell. 2020 Feb 10;37(2):200–215.e5. doi: 10.1016/j.ccell.2020.01.001

Figure 5. AURKB Promotes Murine Myc-Induced T-ALL.

Figure 5.

(A) Zebrafish embryos were injected respectively with the rag2:EGFP-Myc construct alone or in combination with rag2:AURKB. Representative images of GFP fluorescent microscopy analysis at 8 dpf are shown on the left. GFP fluorescence from the thymuses of EGFP-Myc (n = 13) and EGFP-Myc;AURKB fish (n = 14) were qualified and plotted on the right. Scale bar, 1 mm. Data are means ± SD, **p < 0.01, unpaired two-tailed Student’s t test.

(B) Representative images of GFP or mCherry dissemination at 80 dpf in EGFP-Myc;mCherty (n = 10) and EGFP-Myc;mCherry;AURKB (n = 12) transgenic fish receiving heat shock treatments. Scale bar, 1 mm.

(C) Representative GFP images of zebrafish injected with indicated constructs (left). Scale bar, 1 mm. Quantifications of the GFP fluorescence are shown on the right (n = 6 in each group). Data are mean ± SD, **p < 0.01, two-way ANOVA test followed by Tukey’s correction.

(D) Time-course analysis of exogenous murine Myc from zebrafish expressing various transgenes as indicated. The arrow denotes human AURKB transgene expression and the bands below (*) are endogenous zebrafish Aurkb. Zebrafish Actin was used as a loading control (left). Myc protein levels at each point were quantified and plotted on the right.

See also Figure S4.