Skip to main content
. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: Cancer Cell. 2020 Feb 10;37(2):200–215.e5. doi: 10.1016/j.ccell.2020.01.001

Figure 6. AURKB Inhibition Induces Apoptosis in T-ALL Cells Expressing WT FBXW7.

Figure 6.

(A) Analysis of apoptotic cell death by Annexin V/PI staining in normal bone marrow cells and a panel of T-ALL cell lines, harboring WT or mutant FBXW7, treated with AZD1152 for 48 h.

(B) Immunoblots of MYC in 10 nM AZD1152-treated T-ALL cells.

(C) Cell death analysis in MYC S67D expressing KOPTK1 cells (left), MYC-depleted Jurkat cells (middle), or FBXW7-depleted CUTLL1 cells (right), which were subjected to 5, 25, or 5 nM AZD1152 treatment, respectively.

(D) High-throughput screening of US Food and Drug Administration-approved drugs in KOPTK1 cells to identify small molecules synergistic with AZD1152. Top hits are listed on the right.

(E) Assessment of cell death in T-ALL cells treated with AZD1152 (5 nM) and/or vincristine (1 nM) for 48 h. Combo, combination treatment.

Data shown represent the means (±SD) of three biological replicates, *p < 0.05, **p < 0.01; significance was determined by one-way (A and E) or two-way (C) ANOVA test followed by Tukey’s correction. See also Figures S5 and S6 and Table S2.