Figure 5.

Fibroblasts Activated by lncRNA POU3F3 Enhances the Migration and Invasion Abilities of ESCC Cells

(A) Transwell assay of KYSE450 and TE12 cells treated with the indicated CM. Scale bars, 50 um. Results are present as mean ± SD. (B) Wound healing assay of KYSE450 and TE12 cells treated with the indicated CM. (C) qRT-PCR analysis of ICAM-1, VEGF-C, MMP2, MMP3, and MMP9 expression in KY450 and TE12 cells treated with CM/activated fibroblast. (D) Western blot analysis shows MMP2 overexpression in KY450 and TE12 cells treated with CM/activated fibroblast. ∗p < 0.05. ns, not significant