Table 3.
Quantification of TH-IR neurons and fiber densities in GAD67+/+ and GAD67+/GFP mice
| TH-IR neurons and fiber densities | Statistical analysis | ||||||
|---|---|---|---|---|---|---|---|
| TH-IR neurons | Region | Layer | GAD67+/+ (n = 6) | GAD67+/GFP (n = 6) | REGION/LAYER × GENOTYPE | Effect of GENOTYPE | Post hoc |
| Neuron number [n] | SNC | 494.21 ± 28.55 | 465.96 ± 23.49 | – | |||
| SNR | 41.26 ± 2.61 | 45.77 ± 5.74 | F(1.04, 10.44) = 0.68, n.s | F(1, 10) = 0.44, n.s | – | ||
| SNL | 14.06 ± 1.10 | 11.28 ± 1.08 | – | ||||
| Volume [mm3] | SNC | 0.08 ± 0.00 | 0.08 ± 0.01 | – | |||
| SNR | 0.15 ± 0.01 | 0.14 ± 0.01 | F(2, 20) = 2.61, n.s | F(1, 10) = 2.31, n.s | – | ||
| SNL | 0.01 ± 0.00 | 0.01 ± 0.00 | – | ||||
| Density [n/mm3] | SNC | 6077.67 ± 239.37 | 6031.82 ± 214.57 | – | |||
| SNR | 269.47 ± 11.50 | 334.70 ± 33.74 | F(1.30, 13.03) = 0.12, n.s | F(1, 10) = 0.01, n.s | – | ||
| SNL | 1185.79 ± 61.12 | 1137.79 ± 120.15 | – | ||||
| Neuron number [n] | VTA | 283.46 ± 26.88 | 281.23 ± 10.20 | – | n.s. (Welch’s test) | ||
| Volume [mm3] | VTA | 0.07 ± 0.00 | 0.06 ± 0.00 | n.s. (Welch’s test) | |||
| Density [n/mm3] | VTA | 3977.40 ± 259.93 | 4344.88 ± 114.01 | n.s. (Welch’s test) | |||
| TH-IR fiber densities [μm/μm3] | |||||||
| Dorsal hippocampus | CA1 | Or | 0.06 ± 0.00 | 0.07 ± 0.00 | F(2, 20) = 0.02, n.s | F(1, 10) = 20.85, p < 0.01 | p < 0.001 |
| Rad | 0.08 ± 0.01 | 0.09 ± 0.01 | p < 0.05 | ||||
| LMol | 0.09 ± 0.00 | 0.10 ± 0.01 | p < 0.05 | ||||
| CA3 | Or | 0.08 ± 0.01 | 0.09 ± 0.02 | F(2, 20) = 0.30, n.s | F(1, 10) = 1.35, n.s | – | |
| Rad | 0.11 ± 0.02 | 0.12 ± 0.02 | – | ||||
| LMol | 0.09 ± 0.01 | 0.09 ± 0.01 | – | ||||
| DG | Mol | 0.06 ± 0.00 | 0.08 ± 0.02 | F(1, 10) = 0.27, n.s | F(1, 10) = 6.29, p < 0.05 | n.s | |
| ML | 0.09 ± 0.02 | 0.10 ± 0.01 | n.s | ||||
| Amygdala | BLA | 0.36 ± 0.01 | 0.37 ± 0.01 | F(3, 30) = 0.56, n.s | F(1, 10) = 0.11, n.s | – | |
| CeC | 0.32 ± 0.02 | 0.30 ± 0.01 | – | ||||
| CeM | 0.51 ± 0.01 | 0.50 ± 0.01 | – | ||||
| La | 0.25 ± 0.01 | 0.25 ± 0.01 | – | ||||
Coronal brains slices of GAD67+/+ and GAD67+/GFP mice were investigated for densities of tyrosine hydroxylase (TH)-immunoreactive (IR) neurons in subregions of substantia nigra (SN) and ventral tegmental area (VTA) and densities of TH-IR fibers in the dorsal hippocampus and amygdala. Values represent mean ± SEM. Differences between GAD67+/+ and GAD67+/GFP littermates were evaluated using repeated-measures ANOVAs with LAYER or REGION (two to four levels, depending on the investigated brain region) as the within-subject factors and GENOTYPE (two levels: GAD67+/+ and GAD67+/GFP) as the between-subject factor. The interactions and main effects of GENOTYPE are shown. Post hoc analyses were performed using unpaired t tests (Welch’s test) with Bonferroni–Holm adjustment. Neuron densities in the VTA were analyzed using unpaired t test (Welch’s test). For abbreviations, see list