Fig. 4.

Application of improved NN parameters (Table 3) to predict ∆G°₃₇ of sequence d(CCGTACGG) in solution containing 20 wt% 1,3 PDO and 100 mM NaCl. Each arrow represents one NN set. ∆a_w value of the solution was calculated from the data shown in SI Appendix, Table S6. This duplex is self-complementary, with symmetry factor of 0.4 kcal mol⁻¹.