Table 1.

Thermodynamic parameters for DNA sequences measured under crowding conditions of 40 wt% PEG 200 at 100 mM NaCl and predicted without PEG 200 in 100 mM NaCl solution

No.	Sequence*	Measured in the presence of 40 wt% PEG 200†				Predicted in absence of cosolute‡		Difference between with and without cosolute
		ΔH° (kcal mol−1)	TΔS° (kcal mol−1)	ΔG°37 (kcal mol−1)	Tm§ (°C)	ΔG°37 (kcal mol−1)	Tm§ (°C)	ΔΔG°37 (kcal mol−1)	ΔTm (°C)
NS1	d(GGCAGTTC)¶	−67.4 ± 3.4	−62.3 ± 3.2	−5.1 ± 0.4	30.8	−7.1	42.0	2.0	−11.2
NS2	d(GGTTCAGC)¶	−61.0 ± 3.5	−55.9 ± 3.2	−5.1 ± 0.5	29.4	−7.1	42.0	2.0	−12.6
NS3	d(CGCTGTAG)¶	−64.3 ± 2.7	−58.9 ± 2.6	−5.4 ± 0.4	31.7	−6.9	39.3	1.5	−7.6
NS4	d(CGTGCTAG)¶	−68.1 ± 2.8	−62.9 ± 2.6	−5.2 ± 0.4	31.9	−6.9	39.3	1.7	−7.4
NS5	d(AGTAACGCCAT)¶	−83.3 ± 4.4	−76.4 ± 4.1	−6.9 ± 0.6	38.3	−8.7	50.2	1.8	−11.9
NS6	d(AATGCCGTAGT)¶	−77.8 ± 1.8	−71.1 ± 1.6	−6.7 ± 0.2	37.8	−8.7	50.2	2.0	−12.4
NS7	d(CCATCGCTACC)¶	−93.5 ± 2.5	−85.1 ± 2.3	−8.4 ± 0.4	43.4	−9.8	55.2	1.4	−11.8
NS8	d(CGATGGCCTAC)¶	−95.8 ± 3.2	−87.1 ± 2.9	−8.7 ± 0.4	44.1	−9.8	55.2	1.1	−11.1
NS9	d(CGCTTGTTAC)¶	−76.4 ± 1.9	−70.2 ± 1.8	−6.2 ± 0.3	35.2	−8.2	46.3	2.0	−11.1
NS10	d(CCGTAACGTTGG)¶	−95.0 ± 2.6	−87.3 ± 2.4	−8.7 ± 0.4	44.2	−10.6	59.1	1.9	−14.9
NS11	d(ACTGACTGACTG)¶	−88.4 ± 2.1	−80.4 ± 1.9	−8.0 ± 0.3	42.2	−9.5	51.5	1.5	−9.3
NS12	d(ACTGACTGACTGACTG)¶	−124.9 ± 3.2	−113.0 ± 2.9	−11.9 ± 0.5	51.0	−13.1	61.6	1.2	−10.6
S1	d(GGACGTCC)#	−63.0 ± 2.8	−57.7 ± 2.6	−5.3 ± 0.4	35.4	−7.5	48.0	2.2	−12.6
S2	d(GACCGGTC)#	−64.1 ± 6.0	−58.7 ± 5.5	−5.4 ± 0.8	35.6	−7.5	48.0	2.1	−12.4
S3	d(CGTCGACG)#	−63.9 ± 2.2	−57.8 ± 2.0	−6.1 ± 0.3	39.0	−7.9	53.0	1.8	−14.0
S4	d(CGACGTCG)#	−63.2 ± 3.7	−57.0 ± 3.4	−6.2 ± 0.5	39.3	−7.9	53.0	1.7	−13.7
S5	d(CAAGCTTG)#	−79.7 ± 2.4	−76.1 ± 2.3	−3.6 ± 0.3	28.9	−6.2	37.0	2.6	−8.1
S6	d(CTTGCAAG)#	−72.5 ± 3.4	−68.6 ± 3.2	−3.9 ± 0.4	29.6	−6.2	37.0	2.3	−7.4
S7	d(CGGTACCG)#	−69.5 ± 2.7	−64.9 ± 2.6	−4.6 ± 0.3	32.3	−7.6	45.5	3.0	−13.2
S8	d(CCGTACGG)#	−60.5 ± 5.4	−55.1 ± 5.0	−5.4 ± 0.7	34.7	−7.6	45.5	2.2	−10.8
S9	d(GATCCGGATC)#	−82.5 ± 5.1	−76.8 ± 4.8	−5.7 ± 0.7	37.3	−8.4	52.6	2.7	−15.3
S10	d(GGATCGATCC)#	−77.9 ± 2.2	−71.9 ± 2.0	−6.0 ± 0.3	38.2	−8.4	52.6	2.4	−14.4
S11	d(ATGAGCTCAT)#	−71.8 ± 0.9	−66.8 ± 0.8	−5.0 ± 0.1	34.3	−7.6	43.9	2.6	−9.6
S12	d(ATCAGCTGAT)#	−77.1 ± 2.0	−72.2 ± 1.9	−4.9 ± 0.3	34.0	−7.6	43.9	2.7	−9.9
S13	d(CATAGGCCTATG)#	−86.0 ± 4.3	−79.5 ± 4.0	−6.5 ± 0.6	39.8	−8.8	51.3	2.3	−11.5
S14	d(CTATGGCCATAG)#	−91.9 ± 3.6	−85.1 ± 3.4	−6.8 ± 0.5	40.5	−8.8	51.3	2.0	−10.8
S15	d(GCGAATTCGC)#	−66.9 ± 1.9	−59.9 ± 1.7	−7.0 ± 0.3	43.1	−9.4	55.7	2.4	−12.6
S16	d(AGTCATGACT)#	−69.8 ± 4.3	−65.3 ± 4.0	−4.5 ± 0.5	32.3	−6.9	39.3	2.4	−7.0
S17	d(GACGACGTCGTC)#	−95.0 ± 4.4	−85.9 ± 4.0	−9.1 ± 0.6	48.2	−11.5	64.9	2.4	−16.7
S18	d(ATCGCTAGCGAT)#	−59.8 ± 9.0	−53.1 ± 8.0	−6.7 ± 1.3	43.1	−9.8	53.3	3.1	−10.2
S19	d(GCAAGCCGGCTTGC)#	−96.2 ± 8.2	−84.0 ± 7.9	−12.2 ± 0.4	58.5	−14.1	74.0	1.9	−15.5
S20	d(CGATCGGCCGATCG)#	−90.9 ± 8.5	−79.4 ± 8.3	−11.5 ± 0.3	56.9	−13.8	63.2	2.3	−6.3
S21	d(CATATGGCCATATG)#	−129.4 ± 5.8	−122.2 ± 5.5	−7.2 ± 0.7	40.7	−10.0	55.4	2.8	−14.7
S22	d(CAAGATCGATCTTG)#	−116.4 ± 9.9	−108.2 ± 9.3	−8.2 ± 1.3	44.6	−10.7	55.2	2.5	−10.6
S23	d(CGCGTACGCGTACGCG)#	−126.9 ± 6.7	−113.3 ± 6.0	−13.6 ± 1.0	57.9	−16.9	77.2	3.3	−19.3
S24	d(CGCAAGCCGGCTTGCG)#	−109.4 ± 6.8	−94.6 ± 6.4	−14.8 ± 0.4	64.2	−16.7	79.8	1.9	−15.6

^*DNA duplex consists of denoted DNA strand and complementary DNA strand.

^†All experiments proceeded in buffer containing 100 mM NaCl, 10 mM Na₂HPO₄ (pH 7.0), and 1 mM Na₂EDTA.

^‡∆G°₃₇ values in 100 mM NaCl were calculated from those in 1 M NaCl using our previously proposed relationship (32), and T_m values were calculated from those in 1 M NaCl using the equation proposed by Owczarzy et al. (13). Values in 1 M NaCl were predicted using parameters described by SantaLucia et al. (11).

^§Melting temperatures were calculated for total strand concentration of 100 µM.

^¶Non-self-complementary sequences.

^#Self-complementary sequences. Data were taken from our earlier study (17).