Table 2.
NN parameters for DNA duplexes in 40 wt% PEG 200 and 100 mM NaCl at 37 °C
| Sequence | ΔH°NN (kcal mol−1) | ΔS°NN (cal mol−1 K−1) | ΔG°37 NN (kcal mol−1) |
| d(AA/TT) | −6.5 ± 0.3 | −19.2 ± 0.8 | −0.55 ± 0.07 |
| d(AT/TA) | −9.4 ± 0.3 | −29.4 ± 0.8 | −0.28 ± 0.05 |
| d(TA/AT) | −4.3 ± 0.5 | −13.3 ± 1.3 | −0.16 ± 0.14 |
| d(CA/GT) | −13.1 ± 0.1 | −38.8 ± 0.1 | −1.00 ± 0.05 |
| d(GT/CA) | −9.2 ± 0.1 | −26.8 ± 0.1 | −0.89 ± 0.01 |
| d(CT/GA) | −3.4 ± 0.6 | −7.9 ± 1.6 | −0.91 ± 0.11 |
| d(GA/CT) | −4.9 ± 0.7 | −13.0 ± 2.1 | −0.87 ± 0.06 |
| d(CG/GC) | −6.4 ± 0.7 | −16.1 ± 2.0 | −1.38 ± 0.12 |
| d(GC/CG) | −4.2 ± 0.7 | −9.3 ± 2.0 | −1.31 ± 0.06 |
| d(GG/CC) | −4.0 ± 0.6 | −8.9 ± 2.0 | −1.25 ± 0.03 |
| Initiation per GC | −10.1 ± 0.2 | −35.1 ± 0.5 | 0.76 ± 0.06 |
| Initiation per AT | −2.9 ± 0.3 | −12.7 ± 0.9 | 1.00 ± 0.07 |
| Self-complementary | 0 | −1.4 | 0.40 |
| Non-self-complementary | 0 | 0 | 0 |
Experiments proceeded in 10 mM Na2HPO4, 1 mM Na2EDTA, 100 mM NaCl, and 40 wt% PEG 200 at pH 7.0.